Scientific Writer Beckman Coulter, Inc., Karnataka, India
Disclosure(s):
Anisha Jose, PhD: No financial relationships to disclose
Introduction/Rationale: In a typical flow cytometer, the signal generated by a single cell crossing the focused light beam produces an electrical voltage pulse. Three meta-parameters—height (H), area (A), and width (W) of the pulse—are traditionally used to characterize cells across scatter and fluorescence channels. However, cell clumping and closely spaced events can produce overlapping pulses, leading to coincidence effects that compromise data accuracy
Methods: Standard doublet discrimination using area-versus-height plots often relies on subjective user judgment, underscoring the need for more robust and objective gating strategies.
Results: Standard doublet discrimination using area-versus-height plots often relies on subjective user judgment, underscoring the need for more robust and objective gating strategies.
Conclusion: Results are shown using biological samples and NIST-traceable calibration beads.
