Graduate Student Augusta University Augusta, Georgia, United States
Disclosure(s):
Aderonke Fakayode, MASTERS: No financial relationships to disclose
Introduction/Rationale: Coronary artery disease (CAD) is a leading cause of death worldwide and is increasingly recognized as an immune-mediated disorder. Defining immune alterations in CAD can provide mechanistic insights and therapeutic targets.
Methods: Single-cell multi-omics (RNA- and CITE-seq) was applied to profile peripheral blood mononuclear cells from 39 CAD patients undergoing coronary angiography. After quality control and integration (~416,000 singlet cells), we analyzed major immune lineages, transcriptional and proteomic states, and T-cell receptor repertoires.
Results: Distinct states of T cells, B cells, NK cells, and monocytes were identified. Weighted nearest neighbor analysis revealed subsets with altered activation and checkpoint signatures, including heterogeneous CD4⁺ T-cell populations. TCR sequencing demonstrated oligoclonal expansion of 335 clones by TCRB CDR3 sequences. GLIPH2 clustering revealed enrichment of MHC-II–restricted motifs with reactivity to ApoB epitopes, highlighting antigen-driven T-cell responses as potential contributors to CAD progression and potential targets for precision immunotherapy
Conclusion: Integrating this new dataset with published datasets provides an integrated atlas of immune dysregulation in CAD.
