PhD Candidate Weill Cornell Graduate School / Memorial Sloan Kettering New York, New York, United States
Disclosure(s):
Ian McBain, B.S. Biology: No financial relationships to disclose
Introduction/Rationale: Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease where CD8 T cells eliminate insulin-producing β cells in the pancreatic islets. Yet, MHC class II haplotypes confer the greatest genetic risk for the development of T1D, suggesting a critical role for CD4 T cells. Employing the non-obese diabetic (NOD) mouse model, our lab identified in the pancreatic lymph node (pLN) a stem-like β cell-specific CD8 T cell pool required to initiate and sustain disease: pLN β cell-specific stem-CD8 T cells self-renew and continuously give rise to differentiated progenies which migrate to the pancreas (PA) and eliminate β cells; the pLN stem-CD8 T cell pool is absolutely required to sustain β cell destruction. Given the importance of autoimmune stem-CD8 T cells and the association of MHC class II in T1D pathogenesis, we wanted to understand the role of CD4 T cells in autoimmune CD8 T cell stemness and differentiation.
Methods: We employed the NOD model and longitudinally assessed the phenotypic and functional characteristics of β cell-specific CD4 T cells using flow cytometry, serial transplantation, CRISPR/Cas9-mediated gene editing, and transcriptomic studies.
Results: Our studies reveal, for the first time, how CD4 T cells drive autoimmune CD8 T cell stemness, differentiation, and pathogenicity. We find β cell-specific CD4 T cells in pLN and PA reveal two distinct populations based on their expression of TCF1, a transcription factor critical for stemness and self-renewal. Functional studies identify pLN TCF1hi CD4 T cells as stem-T cell subset needed to drive the generation and maintenance of autoimmune stem-CD8 T cells in pLN, their differentiation into β cell-destroying cytotoxic effector cells, and ultimately T1D.
Conclusion: A unique population of β cell-specific CD4 T cells in pLN is critical for autoimmune CD8 T cell stemness, differentiation and disease. Identifying therapeutic strategies that target autoimmune stem-CD4 T cells could emerge as powerful approaches for the treatment of T1D.
